Imininingwane Esheshayo
Uhlelo lokuthola i-PCR lwe-96-well fluorescent quantitative luvumela ukutholwa kwesikhathi sangempela kwe-DNA ekhulisiwe.
Izindawo zokufaka izicelo zihlanganisa ucwaningo ngofuzo lomuntu, i-forensics, umdlavuza, izicubu, isayensi yabantu, i-paleeontology, isayensi yezilwane kanye nesayensi yezitshalo kanye nasekuxilongweni kwezifo zegciwane, umdlavuza nezakhi zofuzo.
Uhlelo lokuthola i-PCR lungolomshini wezokwelapha we-IVD, okuwukusebenzisa i-polymerase chain reaction ukuze kwenziwe ukuhlaziya okulinganiselwe kwezakhi zofuzo ezehlukene elabhorethri yomtholampilo.
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Imininingwane
Umshini oshibhile we-fluorescent quantitative PCR AMPCR04 Applications
Uhlelo lokuthola i-PCR lwe-96-well fluorescent quantitative luvumela ukutholwa kwesikhathi sangempela kwe-DNA ekhulisiwe.
Izindawo zokufaka izicelo zihlanganisa ucwaningo ngofuzo lomuntu, i-forensics, umdlavuza, izicubu, isayensi yabantu, i-paleeontology, isayensi yezilwane kanye nesayensi yezitshalo kanye nasekuxilongweni kwezifo zegciwane, umdlavuza nezakhi zofuzo.
Isistimu yokuthola i-PCR ingeyemishini yezokwelapha ye-IVD, okuwukusebenzisa i-polymerase chain reaction ukuze kwenziwe ukuhlaziya kwenani lezakhi zofuzo ezehlukene elabhorethri yomtholampilo.
Izici zomshini we-PCR womthamo we-fluorescent we-AMPCR04
Inoveli kanye nesixhumi esibonakalayo esisebenzayo esigxile kumuntu ukuze kusebenze kahle.
Imodi yokutholwa yesikhathi sangempela ye-fluorescent itholwa kanyekanye
ukukhulisa nokutholwa kushubhu efanayo ngaphandle kwesidingo sokwelashwa kwangemuva kokuhlolwa.
Ubuchwepheshe be-thermoelectric obuthuthukisiwe buqinisekisa ukushisisa okushesha futhi okuzinzile kanye nokupholisa kwesistimu yamabhayisikili okushisa ashesha kakhulu.
Ukulawula izinga lokushisa kwamaphuzu amaningi kuqinisekisa izinga lokushisa elingaguquki lamasampula emithombo engama-96.
Ingakha i-gradient yezinga lokushisa ngamamojula kagesi we-Thermo angu-4.
Umsebenzi we-gradient ongu-1～36C ozinzile futhi onembile wenza ukulungisa izimo ze-PCR kube lula futhi kube lula.
Umsebenzi oqhubekayo wokushisa we-SOAK unika amandla ukugcinwa kwezinga lokushisa eliphansi lama-reagents e-PCR.
Isebenzisa umthombo wokukhanya we-LED we-excitation wempilo ende engenasondlo.
Ubuchwepheshe bokudluliswa kwe-fiber optic obuthuthukisiwe benza uhlelo lokutholwa kwesithombe-kagesi luzwele kakhulu futhi luthembeke.
Isistimu yendlela yokubona enembile kanye nesistimu ye-PMT ezwela kakhulu ihlinzeka kakhulu
ukutholwa okunembile nokuzwelayo kwe-fluorescent.
Ingakha ukuqapha okuguquguqukayo kwesikhathi sangempela sayo yonke inqubo yokukhulisa i-PCR.
Inomugqa ophezulu wobubanzi obufika kuma-oda ayi-10 wamakhophi okuqala e-DNA ngaphandle kokuhlanjululwa kwe-serial.
Akudingekile ukuvula ishubhu lokusabela le-PCR, ukuqinisekisa ukuthi amasampula avikelekile ekungcoleni phakathi nangemuva kwe-PCR futhi kuqinisekisa imiphumela enembile.
I-Multiplexing iyenzeka.Ubuchwepheshe besivalo esishisayo buvumela ukusebenza ngaphandle kukawoyela kwe-PCR.Ubuchwepheshe besivalo esishisayo esizenzakalelayo abudingi ukuvulwa/ukuvala okwenziwa ngesandla futhi buqinisekisa ukucindezela okungaguquki kwesivalo esishisayo esisetshenziswa ngamashubhu noma amapuleti ahlukene okuphakama.Isixhumi esibonakalayo esisebenziseka kalula ngokulungiselelwa kohlelo oluguquguqukayo nokuhlaziya nokubika kusetshenziswa imingcele egciniwe.
Ingaphrinta umbiko wesampula owodwa noma eminingi.
Amasevisi enethiwekhi azenzakalelayo, alungile futhi afika ngesikhathi anikezela ngosekelo lwakamuva lobuchwepheshe.
Ubuchwepheshe bokutholwa kwemojuli ethuthukisiwe esezingeni eliphansi le-fluorescent eyingqayizivele.
