Zambiri Zachangu
COVID-19 Anti-2020-nCoV Coronavirus Watsopano
zida zoyeserera za coronavirus COVID-19 zida zoyeserera mwachangu za IgM/IgG TUV
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid
Kupaka & Kutumiza
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Zofotokozera
Novel Coronavirus COVID-19 IgG/IgM Diagnostic Rapid AMRPA69
MFUNDO
The COVID-19 IgG/IgM Rapid Test kit (Whole Blood/Serum/Plasma) ndi lateral flow immunochromatographic assay.The
kuyesa kumagwiritsira ntchito anti-anthu IgM antibody (mzere woyesera IgM), anti-munthu IgG (mzere woyesera IgG) ndi mbuzi wotsutsa akalulu IgG (mzere wowongolera C)
osasunthika pamzere wa nitrocellulose.Pad ya burgundy conjugate pad ili ndi golide wa colloidal wolumikizidwa
ma antigen a COVID-19 ophatikizidwa ndi golide wa colloid (COVID-19 conjugates) ndi akalulu a IgG-golide.
Chitsanzo chotsatiridwa ndi buffer choyesa chiwonjezedwa pachitsime, ma antibodies a IgM &/kapena IgG ngati alipo, amamanga
COVID-19 conjugates kupanga antigen antibodies zovuta.Izi zovuta zimasamuka kudzera mu nembanemba ya nitrocellulose
ntchito ya capillary.Pamene zovutazo zikumana ndi mzere wa anti-immobilized antibody (anti-munthu IgM &/kapena
anit-human IgG) chovutacho chatsekeredwa ndikupanga gulu lamtundu wa burgundy lomwe limatsimikizira zotsatira zoyeserera.Kusowa kwa
gulu lachikuda m'chigawo choyesera likuwonetsa zotsatira zoyeserera.
Mayesowa ali ndi chowongolera chamkati (C band) chomwe chikuyenera kuwonetsa gulu lamtundu wa burgundy la mbuzi ya immunocomplex
anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.Apo ayi, mayeso
zotsatira zake ndizolakwika ndipo chitsanzocho chiyesedwenso ndi chipangizo china.
NJIRA YOYESA
Kwa Zitsanzo za Seramu kapena Plasma:
Lolani zida zoyeserera, zoyeserera, zotchingira ndi/kapena zowongolera kuti zigwirizane ndi kutentha kwachipinda (15-30°C) musanayesedwe.
1. Chotsani mzere woyesera/kaseti m'thumba lomata ndikugwiritsa ntchito mwachangu.Zotsatira zabwino zingapezeke ngati
kuyesako kumachitika mkati mwa ola limodzi.
2. Ikani zida zoyesera pamalo oyera komanso osalala.
Mzere: Onjezani 2uL ya seramu / plasma papepala lachitsanzo (malo ofiirira okhala ndi golide wa Colloidal) wa mzere woyeserera, kenaka onjezerani madontho awiri.
(pafupifupi 60 μL) yachitsanzo cha buffer ku buffer pad (pamwamba pa mzere) nthawi yomweyo.
Makaseti:
Onjezani 2uL ya seramu/plasma pachitsime(A) cha makaseti oyesera, kenaka onjezerani madontho awiri (pafupifupi 60 μL) a bafa yachitsanzo ku.
buffer bwino (B) nthawi yomweyo.
3. Dikirani kuti mizere yachikuda iwonekere.Zotsatira zake ziyenera kuwerengedwa kwa mphindi 10.Zotsatira zabwino zitha kuwoneka posachedwa
ngati 2 min.Osatanthauzira zotsatira pambuyo pa mphindi 15
