ʻO ka Novel Coronavirus COVID-19 IgG/IgM Diagnostic wikiwiki

Nā kikoʻī wikiwiki

COVID-19 Anti-2020-nCoV ʻO Coronavirus Hou
pahu hoʻāʻo coronavirus COVID-19 pahu hoʻāʻo wikiwiki IgM/IgG hōʻike TUV
ʻO ka Novel Coronavirus COVID-19 IgG/IgM Diagnostic wikiwiki

Puke a me ka lawe ʻana

Nā kikoʻī

AMRPA68
ʻO COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
INOA MEA KŪʻAI
ʻO COVID-19 IgM/IgG Antibody Rapid Test Kit
(Immunochromatography)
KA MANAO ANA
Hoʻohana ʻia ka reagent e ʻike i ka Corona Virus-19 IgM/IgG Antibody i loko
serum/plasma/ke koko holoʻokoʻa qualitatively.
KUMU HOAO
Hoʻokumu ʻia kēia kit ma ke kumu o ka hōʻike immunochromatographic label gula a hoʻohana i ke ala hopu e ʻike ai i ka antibody COVID-19 IgM/IgG i ka laʻana.

KUMU HOAO

COVID-19 IgM
Ke loaʻa i ka laʻana ka antibody COVID-19 IgM, hana ia i mea paʻakikī me ka antigen lepili gula (COVID-19 recombinant antigen).Ke neʻe nei ka paʻakikī ma lalo o ka hana o ka chromatography a hui pū me ka antibody i uhi ʻia (Mouse anti-human IgM monoclonal antibody) ma ka laina T e hana i kahi paʻakikī a hoʻomohala i ke kala (laina T), he hopena maikaʻi.Inā ʻaʻole i loko o ka laʻana ka antibody COVID-19 IgM, ʻaʻole hiki ke hoʻokumu ʻia ka paʻakikī ma ka laina T, a ʻaʻohe ʻike ʻulaʻula, ʻo ia ka hopena maikaʻi ʻole.
ʻO ka manaʻo o ka COVID-19 IgM antibody i loko o ka laʻana, e hoʻopaʻa ʻia ka antibody control quality label gula (rabbit IgG antibody) me ka antibody coated (goat anti-rabbit IgG antibody) ma ka laina C e hana i kahi paʻakikī a hoʻomohala. kala (laina C).

COVID-19 IgG
Ke loaʻa i ka laʻana ka antibody COVID-19 IgG, hana ia i mea paʻakikī me ka antigen lepili gula (COVID-19 recombinant antigen).Ke neʻe nei ka paʻakikī ma lalo o ka hana o ka chromatography a hui pū me ka antibody i uhi ʻia (Mouse anti-human IgG monoclonal antibody) ma ka laina T e hana i kahi paʻakikī a hoʻomohala i ke kala (laina T), he hopena maikaʻi.Inā ʻaʻole i loko o ka laʻana ka antibody COVID-19 IgG, ʻaʻohe paʻakikī e hiki ke hoʻokumu ʻia ma ka laina T, a ʻaʻohe ʻike ʻulaʻula, ʻo ia ka hopena maikaʻi ʻole.
 

ʻO ka manaʻo o ka COVID-19 IgG antibody i loko o ka laʻana, e hoʻopaʻa ʻia ka antibody control quality label gula (rabbit IgM antibody) me ka antibody coated (goat anti-rabbit IgG antibody) ma ka laina C e hana i kahi paʻakikī a hoʻomohala. kala (laina C).

NA MEA NUI
COVID-19 IgM: T-line i uhi ʻia me ka ʻiole anti-human IgM monoclonal antibody, pad label gula paʻa paʻa COVID-19 recombinant antigen, rabbit IgG antibody, C-line i uhi ʻia me ke kao anti-rabbit IgG antibody.

COVID-19 IgG: T-laina i uhi ʻia me ka ʻiole anti-kanaka IgG monoclonal antibody, ka papa inoa gula paʻa paʻa COVID-19 recombinant antigen, rabbit IgM antibody, C-line i uhi ʻia me ka anti-rabbit IgM antibody.Laʻana hoʻoheheʻe: haku ʻia me 20 mM phosphate buffer solution (PBS)

Waihona a pau
E kūʻai e like me ka mea i hoʻopaʻa ʻia i loko o ka ʻeke i hoʻopaʻa ʻia ma 4-30 ℃, pale i ka wela a me ka lā, kahi maloʻo, kūpono no 12 mau mahina.MAI HOOMAIKAI.Pono e mālama ʻia kekahi mau hana pale i ke kauwela wela a me ka hoʻoilo i mea e pale aku ai i ka wela kiʻekiʻe a i ʻole ka hau hau.Mai wehe i ka pahu i loko a hiki i ka mākaukau, pono e hoʻohana ʻia i hoʻokahi hola inā wehe ʻia (Humidity≤60%, Temp: 20 ℃-30 ℃).E ʻoluʻolu e hoʻohana koke i ka wā o ka haʻahaʻa> 60%.

KAI HOIKE
1. Hiki ke hoʻohana ʻia ka reagent no ka serum, plasma a me ke koko holoʻokoʻa.
2. Pono e hōʻiliʻili ʻia kahi serum / plasma / koko holoʻokoʻa i loko o kahi pahu maʻemaʻe a maloʻo.Hiki ke hoʻohana ʻia ʻo EDTA, sodium citrate, heparin ma ke ʻano he anticoagulants i ka plasma / holoʻokoʻa koko.E ʻike koke ma hope o ka ʻohi ʻana i ke koko.
3. Hiki ke mālama ʻia nā laʻana serum a me ka plasma ma 2-8 ℃ no 3 mau lā ma mua o ka hoʻāʻo ʻana.Inā hoʻopaneʻe ʻia ka hoʻāʻo ʻana ma mua o 3 mau lā, pono e hoʻopaʻa ʻia ka hāpana (-20 ℃ a i ʻole ke anuanu).E hana hou i ka maloo a hoʻoheheʻe ʻia no 3 mau manawa.Hiki ke mālama ʻia nā laʻana koko holoʻokoʻa me ka anticoagulant ma 2-8 ℃ no 3 mau lā, ʻaʻole pono e maloʻo;Pono e hoʻohana koke ʻia nā laʻana koko holoʻokoʻa me ka ʻole o ka anticoagulant (inā he agglutination ka hāpana, hiki ke ʻike ʻia e ka serum).

NA HANA HO'O
Pono e heluhelu ʻia nā ʻōlelo aʻoaʻo ma mua o ka hoʻāʻo ʻana.E ʻae i nā mana hoʻāʻo e hoʻohālikelike i ka lumi wela no 30 mau minuke (20 ℃-30 ℃) ma mua o ka hoʻāʻo ʻana.Mai wehe i ka pahu i loko a hiki i ka mākaukau, pono e hoʻohana ʻia i hoʻokahi hola inā wehe ʻia (Humidity≤60%, Temp: 20 ℃-30 ℃).E ʻoluʻolu e hoʻohana koke i ka wā o ka haʻahaʻa> 60%.
No ka Serum / Plasma
1. Wehe i ka mea ho'āʻo mai ka ʻeke i hoʻopaʻa ʻia, e kau ma luna o kahi maʻemaʻe a paʻa i luna me ka pūnāwai i luna.
2. E hoʻohui i hoʻokahi (1) kulu piha o ka serum a i ʻole ka plasma (10μl) ma ke kū pololei i loko o ka pūnāwai hāpana o IgM a me IgG kaawale.
3. E hoʻohui i ʻelua (2) kulu (80-100μl) o ka laʻana paʻa i loko o ka luawai hāpana o IgM a me IgG kaawale.
4. E nānā koke i nā hopena hōʻike i loko o 15 ~ 20 mau minuke, ʻaʻole kūpono ka hopena ma mua o 20 mau minuke

COVID-19 IgG
Ka nānā ʻana o ka helu coincidence o COVID-19 IgG Ab hoʻāʻo wikiwiki a me ka nucleic acid reagent i nā laʻana serum:
Laki kūpono kūpono=46 / (46+4) × 100% = 92%,
Laki like ʻole ʻino=291 / (9+291) × 100% = 97%,
Ka huina hui like=(46+291) / (46+4+9+291) × 100% = 96.3%.

COVID-19 IgM
Ka nānā ʻana o ka helu coincidence o COVID-19 IgM Ab hoʻāʻo wikiwiki a me ka waikawa nucleic
reagent i nā laʻana serum:
Laki kūpono kūpono=41 / (41+9) × 100% = 82%,
Laki like ʻole ʻino=282 / (18+282) × 100% = 94%,
Huina hui like=(41+282) / (41+9+18+282) × 100% = 92.3%

HOOLAHA
1. No ka hoʻohana diagnostics IN VITRO wale nō.
2. Pono e hoʻohana koke ʻia nā reagents ma hope o ka wehe ʻana.ʻAʻole hiki ke hoʻohana hou ʻia kēia reagent no ka hoʻopau ʻana.
3. Pono ka mea ho'āʻo e noho i loko o nā ʻeke i hoʻopaʻa ʻia a hiki i ka hoʻohana ʻana.Inā pilikia ka sila, mai ho'āʻo.Mai hoʻohana ma hope o ka lā pau.
4. Pono e noʻonoʻo ʻia nā mea hoʻohālike a me nā reagents he mea weliweli a mālama ʻia ma ke ʻano like me kahi mea hoʻopili ma hope o ka hoʻohana ʻana.